LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer

LncRNA MCM3AP-AS1 sponges miR-148a to enhance cell invasion and migration in small cell lung cancer

Blog Article

Abstract Background MCM3AP-AS1 is a recently characterized lncRNA playing an oncogenic role in several cancers.However, its role in lung cancer remains unknown.Here, we aimed to explore the functions of MCM3AP-AS1 in small cell lung cancer (SCLC) and the possible underlying mechanisms.

Methods MCM3AP-AS1 and ROCK1 levels in SCLC patients were analyzed by eeboo coupons qPCR.RNA pull-down and luciferase assays were performed to analyze the interaction between MCM3AP-AS1 and miR-148a.ROCK1 mRNA and protein levels were detected by qPCR and Western blot, respectively.

Cell invasion and migration were analyzed by Transwell assays.Results MCM3AP-AS1 was upregulated in patients with SCLC, and a high MCM3AP-AS1 level was accompanied by a low survival rate.The binding of MCM3AP-AS1 to miR-148a predicted by bioinformatics analysis was verified by RNA pull-down and luciferase assays.

However, MCM3AP-AS1 and miR-148a did not affect each other’s expression.ROCK1 was upregulated in SCLC tissues and positively correlated with MCM3AP-AS1.In SCLC cells, MCM3AP-AS1 overexpression increased ROCK1 and promoted cancer cell invasion and migration, while miR-148a icon track bar f250 overexpression showed the opposite effects and attenuated the effects of MCM3AP-AS1 overexpression on ROCK1 expression and cell behaviors.

Conclusions MCM3AP-AS1 sponges miR-148a, thereby increasing SCLC cell invasion and migration via upregulating ROCK1 expression.